Tmre facs
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Tmre facs
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WebApr 11, 2024 · After 24hrs media was replaced with 200 nM TMRE in serum-free media and incubated for 15min followed by a PBS wash. Live cell imaging was done using ZEISS AXIOLAB 5 Microscope, whereas for FACS analysis cells were trypsinized after treatments and resuspended in PBS for FACS analysis using PI filter (Wang et al., 2024). 2.14. WebJan 1, 1999 · The use of TMRM and TMRE as indicators of mitochondrial membrane potential has become more popular largely because of claims that, unlike tetraphenylphosphonium, these indicators do not exhibit appreciable binding to mitochondria (Loew et al., 1993, Farkas et al., 1989). These indictors have been used to …
WebFluorescence-activated cell sorting (FACS) offers a high-throughput means to quantify fluorescent indicators for a variety of cell and tissue applications. It has long been used to … WebFeb 17, 2024 · The world’s largest grand piano was built by a 15-year-old in New Zealand. The piano is a little over 18 feet long and has 85 keys – 3 short of the standard 88. The …
WebFigure 1. Induction of glycolysis by hypoxia is dependent on growth factor signals. (A) Cells were cultured in the presence or absence (3 wk) of IL-3.Following 24 h incubation at 21% O 2 or 1.5% O 2, cellular glucose utilization was measured by the conversion of 5-3 H-glucose to 3 H 2 O. Data represent mean of three independent experiments ± SD. (B) Glucose … WebTMRM and TMRE signals can be detected in live cells or isolate mitochondria with traditional and high content fluorescence microscopy, microplates, or by flow cytometry (Figure 2). …
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