2024 Sp6 rna polymerasemediated transcription试剂盒

Sp6 rna polymerasemediated transcription试剂盒

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August undefined, 2024

WebSP6 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits high specificity for the bacteriophage SP6 promoter sequence 5'-TAATCCACTGTGATAT-3'. The enzyme can incorporate labeled or unlabeled ribonucleoside triphosphates into an RNA transcript. WebSP6 RNA Polymerase is a DNA-dependent RNA polymerase with high sequence specificity for SP6 promoter sequences. SP6 RNA polymerase synthesizes RNA 5' to 3' and …

New England Biolabs (UK) Ltd - SP6 RNA Polymerase

Web25. nov 1993 · Abstract. SP6 DNA-dependent RNA polymerase, like T7 RNA polymerase, can be used to synthesize RNA sequences from short DNA templates which contain the 18 base pair promoter region. Use of SP6 polymerase extends the range of possible 5' sequences of RNA products, since the preferred SP6 start site (of the RNA product) is 5'GAAGA, while … WebSP6 or T7 RNA polymerase promoter. This DNA produces a transcript approximately 1,800 bases long. Since luciferase must be full-length to show activity, transcription and translation of the Control DNA followed by a luciferase assay is a convenient means to verify that full-length transcripts have been generated.! country othis

What is the promoter sequence of SP6 RNA Polymerase? NEB

WebSP6 RNA polymerase is function tested in the SP6/T7 Transcription Kit (Cat. No. 10 999 644 001). The incorporation rate in the standard assay with 0.5 μg pST18 neo DNA linearized with Eco RI and 50 mCi [alpha-32P] CTP, [400 Ci/mmol (15 TBq/mmol)] gives >50% of the input radioactivity in 20 minutes. 규격 WebSP6 High Yield RNA Transcription Kit. 100次. 1625.00元. 碧云天生产的SP6 High Yield Transcription Kit，即SP6高产量RNA转录试剂盒，是一种利用SP6 RNA Polymerase能在 … WebTRANSCRIPTION BY T7 AND SP6 RNA POLYMERASES VOL. 24, NO. 21, 1985 5717 FIGURE 1: Structure of pT7SP6 DNA. The two bacteriophage promoters are directed toward the same polylinker, but from opposite ... country osu

碧云天-R7020S-SP6 High Yield RNA Transcription Kit（SP6高产 …

Sp6 rna polymerasemediated transcription试剂盒

SP6 RNA Polymerase from Escherichia coli BL 21/pSR3

WebHigh Quality & Proven Performance in RNA In Vitro Transcription Reactions Product Overview: SP6 RNA Polymerase is a recombinant protein from SP6 bacteriophage produced in E.coli. Highly specific for the SP6 promoter that makes it distinct from those of analogous enzymes. It catalyzes efficiently the synthesis of RNA from double-stranded DNA. WebThe RNA Polymerase is rigorously tested for contaminating nonspecific endonuclease, exonuclease, protease, and RNase activity. Functionality of enzyme is tested in an in vitro transcription reaction under optimal and …

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WebBacteriophage SP6 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the SP6 phage promoter. The 98.5 KD polymerase catalyzes in vitro RNA synthesis from a cloned DNA template under the SP6 promoter. RNA synthesized using the SP6 RNA Polymerase is suitable for many applications in research and biotechnology. … WebAssay Time: 145minutes Sample Materials Linearized plasmid DNA; PCR product ; Principle The DIG RNA Labeling Kit produces DIG-labeled, single-stranded RNA probes of known …

WebDocuments. This cloned high-purity Ambion™ SP6 RNA Polymerase has a high specificity for its promoter. The enzyme will transcribe large amounts of RNA from DNA sequences downstream of the promoter and show no … Web30. jan 2015 · SP6 RNA polymerase starts transcription at the underlined G in the promoter sequence. The polymerase then transcribes using the opposite strand as a template from …

WebSP6 RNA Polymerase酶试剂盒Takara. 本酶是鼠伤寒沙门氏菌LT2的噬菌体SP6 DNA编码的酶，分子量为96,000。. 本酶以含有SP6启动子序列的双链DNA为模板，以NTP为底物，合 … WebSP6 体外转录试剂盒数量 25 次反应促进剂 SP6 运输条件干冰产量 15-25 µg 原始材料 PCR 产物、线性化 DNA 质粒内容与储存 SP6 酶混合物、10X 反应缓冲液、2XNTP⁄CAP 溶液 …

WebRNA synthesized using the SP6 RNA Polymerase is suitable for many applications in research and biotechnology. Reaction Conditions: 1X RNAPol Reaction Buffer, … E. COLI Poly(A) Polymerase - SP6 RNA Polymerase NEB RNase Inhibitor, Murine - SP6 RNA Polymerase NEB RNA Loading Dye, (2X) is conveniently supplied in 4 tubes. The dye can be … The ssRNA Ladder is a set of 7 RNA molecules produced by in vitro … The dsRNA Ladder is a set of 7 annealed double-stranded RNA molecules … In vitro synthesis of single-stranded RNA molecules is a widely used laboratory … Rna Synthesis Products - SP6 RNA Polymerase NEB

Web2. feb 2024 · The transcription of mRNA when a mixture of UTP and m 1 ΨTP is present to compete for the SP6 RNA polymerase active site shows minimal sequence context impact on the yield. ( A ) Model of a transcription bubble to illustrate the DNA-protein interactions involved in NTP selection. country osu mapsWebT7 RNA Polymerase is a DNA-dependant RNA Polymerase that exhibits a very high specificity for the T7 promoter sequence. The polymerase is useful for synthesizing large amounts of RNA suitable for in vitro translation and anti-sense RNA research. It can also produce highly sensitive radiolabeled RNA transcripts suitable for hybridization probes. country or us commonwealthWeb碧云天生产的SP6 High Yield Transcription Kit，即SP6高产量RNA转录试剂盒，是一种利用SP6 RNA Polymerase能在相对较短时间内进行体外RNA大量转录的特性而研发的RNA体外 … brewers 4 pack holidayWebDNA-dependent RNA polymerase that catalyzes the transcription of viral genes. Catalytic activity a ribonucleoside 5'-triphosphate + RNA (n) = diphosphate + RNA (n+1) PROSITE-ProRule annotation 1 publication EC:2.7.7.6 ( UniProtKB ENZYME Rhea) Source: Rhea 21248 Hide Rhea reaction a ribonucleoside 5'-triphosphate CHEBI:61557 zoom + RNA(n) brewers 414 ticketsWebSP6 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from an SP6 promoter. The synthesis can be performed with labeled NTPs to generate … country österreichWeb11. okt 2005 · Transcription factor Sp6. Gene. SP6. Status. UniProtKB reviewed (Swiss-Prot) Organism. Homo sapiens (Human) Amino acids. 376. Protein existence. ... DNA-binding transcription factor activity, RNA polymerase II-specific Source:NTNU_SB. Molecular Function: metal ion binding Source:UniProtKB-KW. country oturma grubuWeb21. dec 2016 · The SP6 promoter sequence is 5´ ATTTAGGTGACACTATAG 3´. SP6 RNA Polymerase starts transcription at the underlined G in the double-stranded promoter … brewers 50th anniversary patch