Maize single cell rna-seq
WebJun 26, 2024 · In recent times, scRNA-seq has been utilized to dissect the transcriptome profile of stomatal lineage cell development in Arabidopsis (Liu et al., 2024b), to decipher the transcriptional landscape of maize stem-cell reorganization at single-cell resolution (Satterlee et al., 2024) and also in functional analysis and gene discovery in the ... WebDec 1, 2024 · Single-cell RNA-seq of maize root tips and identification of cell types To perform scRNA-seq on maize inbred line B73, sterilized seeds were germinated with nitrate (nitrate+) or without nitrate (nitrate-) for four days. The primary root length was greater under N-deficit than under N-sufficient conditions (Fig. S1).
Maize single cell rna-seq
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WebDec 14, 2024 · The Mount Sinai researchers used single cell transcriptomic analysis of cell type diversity and lineage choice, followed by validation in primary human autopsy tissue to uniquely capture the transience, diversity and lineage choice of cells as the brain grows in middle and late prenatal development, including novel glial progenitor signatures ... WebApr 28, 2016 · A novel virus, tentatively named Maize Yellow Mosaic Virus (MaYMV), was identified from the field-grown maize plants showing yellow mosaic symptoms on the leaves collected from the Yunnan Province of China by the deep sequencing of small RNAs. The complete 5642 nucleotide (nt)-long genome of the MaYMV shared the highest nucleotide …
WebFeb 4, 2024 · Single-cell RNA sequencing (scRNA-seq) technology has recently been used to examine the cellular identity and heterogeneity of several complex plant tissues in maize ( Zea mays) (Nelms & Walbot, 2024; Satterlee et al ., 2024; Marand et al ., 2024; Xu et al ., 2024 ), Arabidopsis (Denyer et al ., 2024; Zhang et al ., 2024, 2024b; Satterlee et … WebSingle-cell RNA-seq (scRNA-seq) has been highlighted as a powerful tool for the description of human cell transcriptome, but the technology has not been broadly applied in plant cells. ... transcriptional landscape of maize stem-cell reorganization at single-cell resolution (Satterlee et al., 2024) and also in functional
WebFeb 20, 2024 · Background: Maize mesophyll (M) cells play important roles in various biological processes such as photosynthesis II and secondary metabolism. Functional differentiation occurs during M-cell development, but the underlying mechanisms for regulating M-cell development are largely unknown. WebAbstract. Since the first publication a decade ago describing the use of single-cell RNA sequencing (scRNA-seq) in the context of cancer, over 200 datasets and thousands of scRNA-seq studies have been published in cancer biology. scRNA-seq technologies have been applied across dozens of cancer types and diverse array of study designs to …
WebApr 12, 2024 · To fill this knowledge gap, the research team studies the transcriptome age of the nematode C. elegans at the single cell level using RNA sequencing. They look at RNA expression from both whole embryos (or organism) and individual cells to gain a comprehensive understanding of how different genes are used during embryonic and …
WebNov 16, 2024 · Single-cell genomics has slowly advanced in plant research. Here, we introduce a generic procedure for plant nuclei isolation and nanowell-based library preparation for short-read sequencing. This plant-nuclei sequencing (PN-seq) method allows for analyzing several thousands of genes in thousands of individual plant cells. In … jeff martin lawyer virginiaWebSingle-cell RNA sequencing of developing maize ears facilitates functional analysis and trait candidate gene discovery Highlights • scRNA-seq of developing maize ears reveals major cell types and developmental markers • scRNA-seq co-expression networks predict genetic redundancy • jeff martin tenorWebNov 12, 2015 · Analysis of RNA-seq datasets revealed potential transcription associated ... expand exclusively toward a single direction, either in the short or in the long arm, suggesting that ... Genome-wide and organ-specific landscapes of epigenetic modifications and their relationships to mRNA and small RNA transcriptomes in maize. Plant Cell 21 ... jeff martin tribal planetWebJan 1, 2024 · High-throughput single-cell RNA sequencing (scRNA-Seq) offers huge potential to plant research. ... Curiously, Satterlee et al. [19] describe a markedly improved cell viability of isolated maize meristem cells by supplementing protoplast isolation solutions with l-arginine and increasing the pH from a fairly standard 5.7 to 7.5. For select ... oxford m25WebJan 4, 2024 · To ask if scRNA-seq data was required for these insights, we also calculated a list of “whole ear” specific genes from bulk tissue RNA-seq data (Walley et al., 2016), and found a low overlap with our scRNA-seq markers (4%, Table S3), indicating that the bulk tissue RNA-seq lacked cell or developmental domain specific information, as expected. jeff martin pipcornWebMay 7, 2024 · Using single-cell genomics in six maize organs, we determined the cis - and trans -regulatory factors defining diverse cell identities and coordinating chromatin organization by profiling … jeff martin the kingdom lyricsoxford m2r