Hela vimentin
WebAfter being transfected and screened with puromycin, it was found that compared with the HeLa cell controls, both mRNA level and protein expression of vimentin in the transfected HeLa cells were significantly decreased ( Figure 1 B,C), and vimentin knocked out cells were named KO-vimentin HeLa cells, abbreviated as KO HeLa cells. WebJun 10, 2011 · HeLa cell cytospins spiked in blood from healthy volunteers and processed as per patient samples were used as positive controls for the detection of Twist and vimentin. Positive and negative controls for Twist, vimentin and CK are shown in Figure 1.
Hela vimentin
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WebAug 21, 2015 · Vimentin, the major intermediate filament of mesenchymal cells, is mainly involved in tissue integrity and cytoarchitecture 1. The evolutionarily highly conserved protein consists of a central... WebVimentin was one of the CCAT1 binding proteins and was participated in the tumor metastasis pathway. Knocked down VIM and rescued the downregulation by overexpressing CCAT1 demonstrated that CCAT1 could enhance tumor migration and invasion abilities by stabilizing Vimentin protein.
WebWestern blot analysis of extracts from HeLa cells, untreated or phosphorylated in vitro by PLK, using Phospho-Vimentin (Ser83) Antibody (upper). β-Actin Antibody #4976 (lower) was used as a loading control. Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP ® Rabbit mAb. Webab154207 staining Vimentin in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab154207 at a working dilution of 1 in 100 (shown in pseudo color red) ...
WebDec 1, 1992 · Using both vimentin-expressing (HeLa) and vimentin-negative (MCF-7) epithelial cell lines, we have identified the cis-regulatory DNA elements involved in this process. Sequences located 1.1-0.6 kb upstream of the vimentin transcription-initiation site strongly enhance expression in HeLa cells, but are silenced in MCF-7 cells. WebData show that over-expression of vimentin ameliorated cytotoxicity of gambogic acid (GA) in HeLa cells. High vimentin and low beta4 integrin protein levels are associated with poor survival in oral squamous carcinoma patients. PTTG2 induces psoriasis by regulating epidermal expression of vimentin and E-cadherin.
WebThis study represents a comprehensive description of a HeLa xenograft model for in vivo investigations, enabling researchers to assess new treatments for cervical cancer. ...
Web廖昱婷碩士論文，分析HURP p725/Vimentin複合體如何調控Golgi complex的形狀與定位，國立暨南國際大學應用化學系生醫碩士班，2024 鄭巧筠碩士論文，Poh1、Vimentin p72和HURP p725形成蛋白質複合體，並且調控MG132所誘導的aggresome形成，國立暨南國際大學應用化學系生醫碩士 ... エンタメビジネス 本WebSimilar results were obtained using bacterially purified His-tagged Rab7a wt and mutant proteins, in order to pull-down endogenous vimentin from HeLa total extracts (Fig. 2 C–D). Indeed, CMT2B-causing Rab7a mutant proteins were able to pull-down vimentin about 2 fold more efficiently than Rab7a wt (Fig. 2 D). エンタメの未来 本WebSimilar results were obtained using bacterially purified His-tagged Rab7a wt and mutant proteins, in order to pull-down endogenous vimentin from HeLa total extracts (Fig. 2 … えんため大賞WebSep 13, 2024 · Vimentin tail is required for correct network distribution. To elucidate the roles of the vimentin tail domain (Fig. 1a) in filament assembly and stress responses in … pantera razorVimentin was one of the CCAT1 binding proteins and was participated in the tumor metastasis pathway. Knocked down VIM and rescued the downregulation by overexpressing CCAT1 demonstrated that CCAT1 could enhance tumor migration and invasion abilities by stabilizing Vimentin protein. pantera reunion 2022WebMar 31, 2024 · At 8 hpi, cells were fixed, nuclei and replication sites were stained with Hoechst and immunostaining was performed to visualise vimentin. In untreated HeLa cells, vimentin was associated with replication sites and newly assembled virions were found throughout the cytoplasm (Figure 3e). As expected, in acrylamide-treated HeLa cells … pantera rindo gifWebHeLa cell lysate was probed with anti-vimentin (clone RV202, Cat. No. 550513) at concentrations of 120 (lane 1), 60 (lane 2) and 30 ng/ml (lane 3). Vimentin is identified … pantera records